Application
Mouse Melk Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Melk antibody detected the Melk protein (arrow).| Product Name | Melk Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Kiaa0175, Pk38 |
|---|---|
| Alternative Names | Melk Kiaa0175 Pk38 Maternal embryonic leucine zipper kinase Protein kinase PK38 Tyrosine-protein kinase MELK |
| Molecular Weight(MW) | 72729 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis |
| Tissue Specificity | Expressed in testis, ovary, thymus, spleen and T-cell. Expressed by neural progenitors: highly enriched in cultures containing multipotent progenitors |
| Cellular Localization | Cell membrane; Peripheral membrane protein (By similarity) |
| Entrez Gene | 17279 |
|---|---|
| Nucleotide Accession | NP_034920.2 |
Application
Mouse Melk Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Melk antibody detected the Melk protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
