Application
Mouse Nek6 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek6 antibody detected the Nek6 protein (arrow).Application
Western blot analysis of Nek6 (arrow) using rabbit polyclonal Mouse Nek6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.Application
Mouse Nek6 Antibody (C-term) (AP13926b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Nek6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nek6; Serine/threonine-protein kinase Nek6; Never in mitosis A-related kinase 6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Nek6 Serine/threonine-protein kinase Nek6 Never in mitosis A-related kinase 6 |
|---|---|
| Molecular Weight(MW) | 35742 Da |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity) Phosphorylates STAT3 |
| Tissue Specificity | This Mouse Nek6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-280 amino acids from the C-terminal region of Mouse Nek6. |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus speckle (By similarity). Cytoplasm, cytoskeleton, centrosome (By similarity) Cytoplasm, cytoskeleton, spindle pole (By similarity). Note=Co- localizes with APBB1 at the nuclear speckles. Co-localizes with PIN1 in the nucleus. Co-localizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis (By similarity) |
| Entrez Gene | 59126 |
|---|---|
| Nucleotide Accession | NP_001153103.1 NP_067619.1 |
Application
Mouse Nek6 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek6 antibody detected the Nek6 protein (arrow).
Application
Western blot analysis of Nek6 (arrow) using rabbit polyclonal Mouse Nek6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.
Application
Mouse Nek6 Antibody (C-term) (AP13926b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
