Application
Mouse Pak1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Pak1 antibody detected the Pak1 protein (arrow).Application
Mouse Pak1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Pak1 antibody detected the Pak1 protein (arrow).| Product Name | Pak1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pak1; Paka; Serine/threonine-protein kinase PAK 1; Alpha-PAK; CDC42/RAC effector kinase PAK-A; p21-activated kinase 1; p65-PAK |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Paka |
|---|---|
| Alternative Names | Pak1 Paka Serine/threonine-protein kinase PAK 1 Alpha-PAK CDC42/RAC effector kinase PAK-A p21-activated kinase 1 p65-PAK |
| Molecular Weight(MW) | 60737 Da |
| Function | Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus (By similarity). Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Can directly phosphorylate BAD and protects cells against apoptosis |
| Tissue Specificity | This Mouse Pak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of Mouse Pak1. |
| Cellular Localization | Cytoplasm (By similarity). Cell junction, focal adhesion (By similarity). Cell membrane (By similarity) Note=Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation (By similarity) |
Application
Mouse Pak1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Pak1 antibody detected the Pak1 protein (arrow).
Application
Mouse Pak1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Pak1 antibody detected the Pak1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
