Application
Mouse Pak3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Pak3 antibody detected the Pak3 protein (arrow).Application
Mouse Pak3 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Pak3 antibody detected the Pak3 protein (arrow).Application
Mouse Pak3 Antibody (N-term) (AP13931a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Pak3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Pak-3, Pakb, Stk4 |
|---|---|
| Alternative Names | Pak3 Pak-3 Pakb Stk4 Serine/threonine-protein kinase PAK 3 Beta-PAK CDC42/RAC effector kinase PAK-B p21-activated kinase 3 |
| Molecular Weight(MW) | 62398 Da |
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments |
| Tissue Specificity | This Mouse Pak3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-152 amino acids from the N-terminal region of Mouse Pak3. |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 18481 |
|---|---|
| Nucleotide Accession | NP_001181977.1 NP_032804.2 |
Application
Mouse Pak3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Pak3 antibody detected the Pak3 protein (arrow).
Application
Mouse Pak3 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Pak3 antibody detected the Pak3 protein (arrow).
Application
Mouse Pak3 Antibody (N-term) (AP13931a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
