Application
Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).Application
Mouse Pak4 Antibody (Center) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).Application
Mouse Pak4 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Pak4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Kiaa1142 |
|---|---|
| Alternative Names | Pak4 Kiaa1142 Serine/threonine-protein kinase PAK 4 p21-activated kinase 4 |
| Molecular Weight(MW) | 64623 Da |
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN |
| Tissue Specificity | This Mouse Pak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-206 amino acids from the Central region of Mouse Pak4. |
| Cellular Localization | Cytoplasm. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways (By similarity) |
| Entrez Gene | 70584 |
|---|---|
| Nucleotide Accession | NP_081746.1 |
Application
Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).
Application
Mouse Pak4 Antibody (Center) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).
Application
Mouse Pak4 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
