Application
Mouse Pak7 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Pak7 antibody detected the Pak7 protein (arrow).Application
Mouse Pak7 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Pak7 antibody detected the Pak7 protein (arrow).Application
Mouse Pak7 Antibody (N-term) (AP14074a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Pak7 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Pak5 |
|---|---|
| Alternative Names | Pak7 Pak5 Serine/threonine-protein kinase PAK 7 p21-activated kinase 5 p21-activated kinase 7 |
| Molecular Weight(MW) | 80948 Da |
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity) |
| Tissue Specificity | Highly expressed in brain and eye. Also expressed in adrenal gland, pancreas, prostate and testes. Within the brain, expression is restricted to neurons. Present in brain but not in kidney, lung and spleen (at protein level) |
| Cellular Localization | Mitochondrion (By similarity). Cytoplasm (By similarity). Nucleus (By similarity). Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival (By similarity) |
| Entrez Gene | 241656 |
|---|---|
| Nucleotide Accession | NP_766446.2 |
Application
Mouse Pak7 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Pak7 antibody detected the Pak7 protein (arrow).
Application
Mouse Pak7 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Pak7 antibody detected the Pak7 protein (arrow).
Application
Mouse Pak7 Antibody (N-term) (AP14074a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
