Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
The anti-Mouse Phospho-Bid-S61 Pab is used in Western blot to detect Mouse Phospho-Bid-S61 in Jurkat tissue lysate| Product Name | Phospho-Bid-S61 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S61 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Bid BH3-interacting domain death agonist p22 BID BH3-interacting domain death agonist p15 p15 BID BH3-interacting domain death agonist p13 p13 BID BH3-interacting domain death agonist p11 p11 BID |
|---|---|
| Molecular Weight(MW) | 21952 Da |
| Function | Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c |
| Tissue Specificity | This Mouse Phospho-Bid-S61 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse Bid. |
| Cellular Localization | Cytoplasm. Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
The anti-Mouse Phospho-Bid-S61 Pab is used in Western blot to detect Mouse Phospho-Bid-S61 in Jurkat tissue lysate| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
