Application
Mouse Pdk2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).Application
Western blot analysis of Pdk2 (arrow) using rabbit polyclonal Mouse Pdk2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pdk2 gene.Application
Mouse Pdk2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).Application
Mouse Pdk2 Antibody (N-term) (AP14075a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pdk2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Pdk2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pdk2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial; Pyruvate dehydrogenase kinase isoform 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Pdk2 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 mitochondrial Pyruvate dehydrogenase kinase isoform 2 |
|---|---|
| Molecular Weight(MW) | 46041 Da |
| Function | Serine/threonine kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis |
| Tissue Specificity | Detected in heart (at protein level). |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 18604 |
|---|---|
| Nucleotide Accession | NP_598428.2 |
Application
Mouse Pdk2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).
Application
Western blot analysis of Pdk2 (arrow) using rabbit polyclonal Mouse Pdk2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pdk2 gene.
Application
Mouse Pdk2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).
Application
Mouse Pdk2 Antibody (N-term) (AP14075a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pdk2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
