Application
Dot blot analysis of anti-Mouse Phospho-TNFR-S299 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
The anti-Mouse Phospho-TNFR-S299 Pab is used in Western blot to detect Mouse Phospho-TNFR-S299 in mouse thymus tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-TNFR-S299 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tnfrsf1a; Tnfr-1; Tnfr1; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor type I; p55; p60; CD_antigen=CD120a; Flags: Precursor |
| Modification | p-S299 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWBIHC |
| DB:1:500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Tnfr-1 Tnfr1 |
|---|---|
| Alternative Names | Tnfrsf1a Tnfr-1 Tnfr1 Tumor necrosis factor receptor superfamily member 1A Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 p60 CD_antigen=CD120a Flags: Precursor |
| Molecular Weight(MW) | 50130 Da |
| Function | Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis (By similarity) |
| Tissue Specificity | This Mouse Phospho-TNFR-S299 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S299 of mouse TNFR. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein (By similarity) |
| Entrez Gene | 21937 |
|---|
Application
Dot blot analysis of anti-Mouse Phospho-TNFR-S299 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
The anti-Mouse Phospho-TNFR-S299 Pab is used in Western blot to detect Mouse Phospho-TNFR-S299 in mouse thymus tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | DB:1:500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
