Application
Mouse Pim1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Pim1 antibody detected the Pim1 protein (arrow).Application
Mouse Pim1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Pim1 antibody detected the Pim1 protein (arrow).| Product Name | Pim1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Pim-1 |
|---|---|
| Alternative Names | Pim1 Pim-1 Serine/threonine-protein kinase pim-1 |
| Molecular Weight(MW) | 44542 Da |
| Function | Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post- translational levels. Phosphorylation of CDKN1B,induces 14-3-3 binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis (By similarity) |
| Tissue Specificity | This Mouse Pim1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-372 amino acids from the C-terminal region of Mouse Pim1. |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Note=Mainly located in the cytoplasm |
Application
Mouse Pim1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Pim1 antibody detected the Pim1 protein (arrow).
Application
Mouse Pim1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Pim1 antibody detected the Pim1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
