Application
Mouse Plk4 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the Plk4 antibody detected the Plk4 protein (arrow).Application
Mouse Plk4 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Plk4 antibody detected the Plk4 protein (arrow).Application
Mouse Plk4 Antibody (C-term) (AP13934b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Plk4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Plk4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Sak, Stk18 |
|---|---|
| Alternative Names | Plk4 Sak Stk18 Serine/threonine-protein kinase PLK4 Polo-like kinase 4 Serine/threonine-protein kinase 18 Serine/threonine-protein kinase Sak |
| Molecular Weight(MW) | 103746 Da |
| Function | Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2 (By similarity). Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1 |
| Tissue Specificity | expressed in tissues associated with mitotic and meiotic cell division. Highly expressed in testis |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome, centriole. Nucleus, nucleolus. Cleavage furrow. Note=Associates with centrioles throughout the cell cycle. According to PubMed:11301255, it localizes to the nucleolus during G2, to the centrosomes in G2/M, and to the cleavage furrow during cytokinesis |
| Entrez Gene | 20873 |
|---|---|
| Nucleotide Accession | NP_035625.2 |
Application
Mouse Plk4 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the Plk4 antibody detected the Plk4 protein (arrow).
Application
Mouse Plk4 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Plk4 antibody detected the Plk4 protein (arrow).
Application
Mouse Plk4 Antibody (C-term) (AP13934b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Plk4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
