Application
Mouse Pkn2 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Pkn2 antibody detected the Pkn2 protein (arrow).| Product Name | Pkn2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Prk2, Prkcl2 |
|---|---|
| Alternative Names | Pkn2 Prk2 Prkcl2 Serine/threonine-protein kinase N2 PKN gamma Protein kinase C-like 2 Protein-kinase C-related kinase 2 |
| Molecular Weight(MW) | 111607 Da |
| Function | PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF) |
| Tissue Specificity | Ubiquitous. Highly expressed in liver and lung Expressed in astrocytes (at protein level). Ubiquitous |
| Cellular Localization | Cytoplasm. Nucleus (By similarity). Membrane. Cell projection, lamellipodium (By similarity) Cytoplasm, cytoskeleton (By similarity). Cleavage furrow (By similarity). Midbody (By similarity). Cell junction (By similarity). Note=Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells (By similarity) |
| Entrez Gene | 109333 |
|---|---|
| Nucleotide Accession | NP_848769.2 |
Application
Mouse Pkn2 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Pkn2 antibody detected the Pkn2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
