Application
Mouse Prkaca/Prkacb Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).Application
Mouse Prkaca/Prkacb Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).Application
Mouse Prkaca/Prkacb Antibody (C-term) (AP14456b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkaca/Prkacb Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Prkaca/Prkacb Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Pkacb |
|---|---|
| Alternative Names | Prkacb Pkacb cAMP-dependent protein kinase catalytic subunit beta |
| Molecular Weight(MW) | 40708 Da |
| Function | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (By similarity) |
| Tissue Specificity | Isoform 1 is found in all tissues examined, with the highest expression in the brain and very low levels in the testis. Isoform 2 is strongly expressed in the brain, in the prelimbic and insular cortex. Isoform 3 is also found only in the brain, but at very low levels |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity) |
| Entrez Gene | 18749 |
|---|---|
| Nucleotide Accession | NP_001157672.1 NP_001157670.1 |
Application
Mouse Prkaca/Prkacb Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).
Application
Mouse Prkaca/Prkacb Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).
Application
Mouse Prkaca/Prkacb Antibody (C-term) (AP14456b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkaca/Prkacb Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
