Application
Mouse Rnasen Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Rnasen antibody detected the Rnasen protein (arrow).| Product Name | Rnasen Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Etohi2, Rn3, Rnasen |
|---|---|
| Alternative Names | Drosha Etohi2 Rn3 Rnasen Ribonuclease 3 Protein Drosha Ribonuclease III |
| Molecular Weight(MW) | 158828 Da |
| Function | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity) |
| Tissue Specificity | This Mouse Rnasen antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-764 amino acids from the Central region of mouse Rnasen. |
| Cellular Localization | Nucleus (By similarity). Nucleus, nucleolus (By similarity). Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies (By similarity) |
| Entrez Gene | 14000 |
|---|---|
| Nucleotide Accession | NP_001123621.1 |
Application
Mouse Rnasen Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Rnasen antibody detected the Rnasen protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
