Application
Western blot analysis of mouse anti-Semaphorin 5A Pab in mouse brain tissue lysates (35ug/lane). Semaphorin 5A(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | Sema5a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SEMAF |
|---|---|
| Alternative Names | SEMA5A SEMAF Semaphorin-5A Semaphorin-F |
| Molecular Weight(MW) | 120615 Da |
| Function | Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3 In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis |
| Tissue Specificity | This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of mouse Sema5a. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 9037 |
|---|
Application
Western blot analysis of mouse anti-Semaphorin 5A Pab in mouse brain tissue lysates (35ug/lane). Semaphorin 5A(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
