Application
Mouse Sgk3 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the Sgk3 antibody detected the Sgk3 protein (arrow).Application
Western blot analysis of Sgk3 (arrow) using rabbit polyclonal Mouse Sgk3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Sgk3 gene.Application
Mouse Sgk3 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Sgk3 antibody detected the Sgk3 protein (arrow).| Product Name | Sgk3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Cisk, Sgkl |
|---|---|
| Alternative Names | Sgk3 Cisk Sgkl Serine/threonine-protein kinase Sgk3 Cytokine-independent survival kinase Serum/glucocorticoid-regulated kinase 3 Serum/glucocorticoid-regulated kinase-like |
| Molecular Weight(MW) | 57145 Da |
| Function | Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes |
| Tissue Specificity | Widely expressed, predominantly in the heart, spleen and 7-day embryo |
| Cellular Localization | Cytoplasmic vesicle. Early endosome. Recycling endosome (By similarity). Note=Endosomal localization is a prerequisite for complete kinase activity. It is essential for its co-localization with the kinase responsible for phosphorylating Ser-486 thus allowing PDPK1 phosphorylation of Thr-320 resulting in complete activation of SGK3. Co-localizes with SLC9A3/NHE3 in the recycling endosomes (By similarity) Localized in vesicle-like structures and in the early endosome |
| Entrez Gene | 170755 |
|---|---|
| Nucleotide Accession | NP_808215.2 NP_573483.1 |
Application
Mouse Sgk3 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the Sgk3 antibody detected the Sgk3 protein (arrow).
Application
Western blot analysis of Sgk3 (arrow) using rabbit polyclonal Mouse Sgk3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Sgk3 gene.
Application
Mouse Sgk3 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Sgk3 antibody detected the Sgk3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
