Application
Western blot analysis of Sirt3 Antibody (C-term) in mouse brain tissue lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab.Application
Western blot analysis of Sirt3 Antibody (C-term) in CHO cell line lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab.Application
Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Sirt3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Sir2l3 |
|---|---|
| Alternative Names | Sirt3 Sir2l3 NAD-dependent protein deacetylase sirtuin-3 Regulatory protein SIR2 homolog 3 SIR2-like protein 3 |
| Molecular Weight(MW) | 28822 Da |
| Function | NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues (By similarity). Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels |
| Tissue Specificity | Strongly expressed in liver and kidney. Weakly expressed in lung |
| Cellular Localization | Cytoplasm. Mitochondrion matrix (Probable). |
| Entrez Gene | 64384 |
|---|---|
| Nucleotide Accession | NP_071878.2 |
Application
Western blot analysis of Sirt3 Antibody (C-term) in mouse brain tissue lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of Sirt3 Antibody (C-term) in CHO cell line lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab.
Application
Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
