Application
Mouse Stk3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Stk3 antibody detected the Stk3 protein (arrow).Application
Western blot analysis of Stk3 (arrow) using rabbit polyclonal Mouse Stk3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Stk3 gene.| Product Name | Stk3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Mess1, Mst2 |
|---|---|
| Alternative Names | Stk3 Mess1 Mst2 Serine/threonine-protein kinase 3 Mammalian STE20-like protein kinase 2 STE20-like kinase MST2 Serine/threonine-protein kinase 3 36kDa subunit Serine/threonine-protein kinase 3 20kDa subunit |
| Molecular Weight(MW) | 56855 Da |
| Function | Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (By similarity) |
| Tissue Specificity | This Mouse Stk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-415 amino acids from the C-terminal region of Mouse Stk3. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Note=The caspase-cleaved form cycles between nucleus and cytoplasm. Phosphorylation at Thr-117 leads to inhibition of nuclear translocation (By similarity) |
| Entrez Gene | 56274 |
|---|---|
| Nucleotide Accession | NP_062609.2 |
Application
Mouse Stk3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Stk3 antibody detected the Stk3 protein (arrow).
Application
Western blot analysis of Stk3 (arrow) using rabbit polyclonal Mouse Stk3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Stk3 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
