Application
Mouse Ulk3 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the Ulk3 antibody detected the Ulk3 protein (arrow).| Product Name | Ulk3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Ulk3 Serine/threonine-protein kinase ULK3 Unc-51-like kinase 3 |
|---|---|
| Molecular Weight(MW) | 53572 Da |
| Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (By similarity) |
| Tissue Specificity | This Mouse Ulk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-262 amino acids from the Central region of Mouse Ulk3. |
| Cellular Localization | Cytoplasm (By similarity). Note=Localizes to pre-autophagosomal structure during cellular senescence (By similarity) |
| Entrez Gene | 71742 |
|---|---|
| Nucleotide Accession | NP_082171.1 |
Application
Mouse Ulk3 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the Ulk3 antibody detected the Ulk3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
