Application
Mouse Vrk2 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Vrk2 antibody detected the Vrk2 protein (arrow).| Product Name | Vrk2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Vrk2 Serine/threonine-protein kinase VRK2 Vaccinia-related kinase 2 |
|---|---|
| Molecular Weight(MW) | 58119 Da |
| Function | Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates histone H3. Phosphorylates 'Thr-18' of p53/TP53, and thereby increases its stability and activity Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. May also phosphorylate MAPK8IP1. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (By similarity) |
| Tissue Specificity | Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum membrane; Single-pass type IV membrane protein (Potential). Mitochondrion membrane; Single-pass type IV membrane protein (Potential) |
| Entrez Gene | 69922 |
|---|---|
| Nucleotide Accession | NP_081536.2 |
Application
Mouse Vrk2 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Vrk2 antibody detected the Vrk2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
