Application
Mouse Wee2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Wee2 antibody detected the Wee2 protein (arrow).Application
Mouse Wee2 Antibody (Center) (AP14472c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse ovarian tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Wee2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Wee2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Wee1b |
|---|---|
| Alternative Names | Wee2 Wee1b Wee1-like protein kinase 2 Wee1-like protein kinase 1B Wee1B kinase |
| Molecular Weight(MW) | 62343 Da |
| Function | Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation |
| Tissue Specificity | Ovary-specific. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Localizes mainly in the nucleus. Exported from the nucleus to the cytoplasm before germinal vesicle breakdown (GVBD), allowing meiosis resumption |
| Entrez Gene | 381759 |
|---|---|
| Nucleotide Accession | NP_958758.2 |
Application
Mouse Wee2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Wee2 antibody detected the Wee2 protein (arrow).
Application
Mouse Wee2 Antibody (Center) (AP14472c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse ovarian tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Wee2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
