Application
A. Western blot analysis on A431 cell lysate using anti-MP1 (C-term) RabMAb (Cat. #AJ1506a), dilution 1:50,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-MP1 (C-term) RabMAb (Cat. #AJ1506a).| Product Name | MP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y130 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAP2K1IP1, MAPKSP1 |
|---|---|
| Alternative Names | MAPKSP1 MAP2K1IP1 Mitogen-activated protein kinase scaffold protein 1 Mitogen-activated protein kinase kinase 1-interacting protein 1 MEK-binding partner 1 |
| Molecular Weight(MW) | 13623 Da |
| Function | As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2 |
| Tissue Specificity | A synthetic peptide corresponding to residues in the C-terminus of human mitogen-activated protein kinase kinase 1 interacting protein 1 was used as immunogen. |
| Cellular Localization | Late endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) |
| Entrez Gene | 8649 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-MP1 (C-term) RabMAb (Cat. #AJ1506a), dilution 1:50,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-MP1 (C-term) RabMAb (Cat. #AJ1506a).| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
