Application
Western blot analysis of Mre11 antibody (Center K475) in Hela cell line lysates (35ug/lane). MRE11A (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of hela cells using Mre11 Antibody (Center K475)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Mre11 Antibody (Center K475) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNGS1, MRE11 |
|---|---|
| Alternative Names | MRE11A HNGS1 MRE11 Double-strand break repair protein MRE11A Meiotic recombination 11 homolog 1 Meiotic recombination 11 homolog A |
| Molecular Weight(MW) | 80593 Da |
| Function | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation |
| Tissue Specificity | This Mre11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467~496 amino acids from the Center region of human MRE11. |
| Cellular Localization | Nucleus (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity) |
| Entrez Gene | 4361 |
|---|
Application
Western blot analysis of Mre11 antibody (Center K475) in Hela cell line lysates (35ug/lane). MRE11A (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of hela cells using Mre11 Antibody (Center K475)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
