Application
MRPL28 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MRPL28 antibody detected the MRPL28 protein (arrow).Application
Fluorescent confocal image of A431 cell stained with MRPL28 Antibody (N-term)(Cat#AP19152a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MRPL28 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MRPL28 immunoreactivity is localized to Mitochondria significantly.| Product Name | MRPL28 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAAT1 |
|---|---|
| Alternative Names | MRPL28 MAAT1 39S ribosomal protein L28 mitochondrial Melanoma antigen p15 Melanoma-associated antigen recognized by T-lymphocytes |
| Molecular Weight(MW) | 30157 Da |
| Tissue Specificity | Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 10573 |
|---|---|
| Nucleotide Accession | NP_006419.2 |
Application
MRPL28 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MRPL28 antibody detected the MRPL28 protein (arrow).
Application
Fluorescent confocal image of A431 cell stained with MRPL28 Antibody (N-term)(Cat#AP19152a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MRPL28 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MRPL28 immunoreactivity is localized to Mitochondria significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
