Application
Western blot analysis on (A) A375, (B) A431, (C) SW480, and (D) HeLa cell lysates using anti-MSH2 RabMAb (Cat. #AJ1509a).Application
Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma using anti-MSH2 RabMAb (Cat. #AJ1509a).Application
Immunofluorescent staining of HeLa cells using anti-MSH2 RabMAb (Cat. #AJ1509a).| Product Name | MSH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3943 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1 : 1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 104743 Da |
|---|---|
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 4436 |
|---|
Application
Western blot analysis on (A) A375, (B) A431, (C) SW480, and (D) HeLa cell lysates using anti-MSH2 RabMAb (Cat. #AJ1509a).
Application
Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma using anti-MSH2 RabMAb (Cat. #AJ1509a).
Application
Immunofluorescent staining of HeLa cells using anti-MSH2 RabMAb (Cat. #AJ1509a).| Application Notes | WB:1 : 1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
