Application
The anti-MTM1 C-term Pab is used in Western blot to detect MTM1 in NCI-H460 cell lysate (lane 1) and in mouse heart tissue lysate (lane 2).Application
Western blot showing knockdown of endogenous MTM1 expression by MTM1-targeting vectors pDM134 and pDM170. Embryonic stem (ES) cells were untreated (lane 1) or transfected with control plasmid pDCont (lane 2), MTM1-targeting plasmid pDM134 (lane 3), or pDM170 (lane 4). The blot was probed with anti-MTM1 rabbit polyclonal antibodies. -Actin was used as a loading control.| Product Name | MTM1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MTM1; CG2; Myotubularin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CG2 |
|---|---|
| Alternative Names | MTM1 CG2 Myotubularin |
| Molecular Weight(MW) | 69932 Da |
| Function | Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine- containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology Regulates desmin intermediate filament assembly and architecture Plays a role in mitochondrial morphology and positioning. Required for skeletal muscle maintenance but not for myogenesis |
| Tissue Specificity | This MTM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573~602 amino acids from the C-terminal region of human MTM1. |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Late endosome. Note=Localizes as a dense cytoplasmic network. Also localizes to the plasma membrane, including plasma membrane extensions such as filopodia and ruffles. Predominantly located in the cytoplasm following interaction with MTMR12 Recruited to the late endosome following EGF stimulation |
| Entrez Gene | 4534 |
|---|
Application
The anti-MTM1 C-term Pab is used in Western blot to detect MTM1 in NCI-H460 cell lysate (lane 1) and in mouse heart tissue lysate (lane 2).
Application
Western blot showing knockdown of endogenous MTM1 expression by MTM1-targeting vectors pDM134 and pDM170. Embryonic stem (ES) cells were untreated (lane 1) or transfected with control plasmid pDCont (lane 2), MTM1-targeting plasmid pDM134 (lane 3), or pDM170 (lane 4). The blot was probed with anti-MTM1 rabbit polyclonal antibodies. -Actin was used as a loading control.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
