Application
Western blot analysis of MTMRF Antibody (N-term) in K562 cell line lysates (35ug/lane).MTMRF (arrow) was detected using the purified Pab.Application
MTMRF Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MTMRF Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FAN1; KIAA1018; MTMR15; Fanconi-associated nuclease 1; FANCD2/FANCI-associated nuclease 1; Myotubularin-related protein 15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1018 MTMR15 |
|---|---|
| Alternative Names | FAN1 KIAA1018 MTMR15 Fanconi-associated nuclease 1 FANCD2/FANCI-associated nuclease 1 Myotubularin-related protein 15 |
| Molecular Weight(MW) | 114225 Da |
| Function | Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL- induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap- specific enzymes to unhook the ICL by cleaving the lagging-strand template |
| Tissue Specificity | This MTMRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-185 amino acids from the N-terminal region of human MTMRF. |
| Cellular Localization | Nucleus. Note=Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2 |
| Entrez Gene | 22909 |
|---|
Application
Western blot analysis of MTMRF Antibody (N-term) in K562 cell line lysates (35ug/lane).MTMRF (arrow) was detected using the purified Pab.
Application
MTMRF Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
