Application
The anti-MVP C-term Antibody is used in Western blot to detect MVP in A375 lysate.Application
Western blot analysis of MVP (arrow) using rabbit polyclonal MVP C-term Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human lung carcinoma with MVP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using MVP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MVP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MVP; LRP; Major vault protein; Lung resistance-related protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LRP |
|---|---|
| Alternative Names | MVP LRP Major vault protein Lung resistance-related protein |
| Molecular Weight(MW) | 99327 Da |
| Function | Required for normal vault structure. Vaults are multi- subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo- cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases |
| Tissue Specificity | Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells |
| Cellular Localization | Cytoplasm. Nucleus, nuclear pore complex. Note=5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment |
Application
The anti-MVP C-term Antibody is used in Western blot to detect MVP in A375 lysate.
Application
Western blot analysis of MVP (arrow) using rabbit polyclonal MVP C-term Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with MVP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using MVP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
