Application
MYLIP Antibody (Center T126) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow)Application
MYLIP Antibody (Center T126) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow).Application
MYLIP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the MYLIP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MYLIP Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MYLIP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MYLIP; BZF1; IDOL; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Myosin regulatory light chain interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BZF1 IDOL |
|---|---|
| Alternative Names | MYLIP BZF1 IDOL E3 ubiquitin-protein ligase MYLIP Inducible degrader of the LDL-receptor Myosin regulatory light chain interacting protein |
| Molecular Weight(MW) | 49910 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Cytoplasm (Probable). Cell membrane; Peripheral membrane protein |
Application
MYLIP Antibody (Center T126) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow)
Application
MYLIP Antibody (Center T126) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow).
Application
MYLIP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the MYLIP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MYLIP Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
