Application
MyoD1 Antibody (Center) (Cat#AP12646c) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow).Application
Fluorescent confocal image of Hela cell stained with MyoD1 Antibody (Center)(Cat#AP12646c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly.| Product Name | MyoD1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; Myogenic factor 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHC1 MYF3 MYOD |
|---|---|
| Alternative Names | MYOD1 BHLHC1 MYF3 MYOD Myoblast determination protein 1 Class C basic helix-loop-helix protein 1 Myogenic factor 3 |
| Molecular Weight(MW) | 34501 Da |
| Function | Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity) |
| Tissue Specificity | This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-247 amino acids from the Central region of human MyoD1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4654 |
|---|---|
| Nucleotide Accession | NP_002469.2 |
Application
MyoD1 Antibody (Center) (Cat#AP12646c) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow).
Application
Fluorescent confocal image of Hela cell stained with MyoD1 Antibody (Center)(Cat#AP12646c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:1000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
