Application
Western blot analysis of MYO1C Antibody (C-term) in K562 cell line lysates (35ug/lane). MYO1C (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MYO1C antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Myosin 1C (MYO1C) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MYO1C; Unconventional myosin-Ic; Myosin I beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MYO1C Unconventional myosin-Ic Myosin I beta |
|---|---|
| Molecular Weight(MW) | 121682 Da |
| Function | Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes (By similarity) |
| Tissue Specificity | This MYO1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 883~913 amino acids from the C-terminal region of human MYO1C. |
| Cellular Localization | Cytoplasm. Cell membrane (By similarity). Cell projection, stereocilium membrane (By similarity) Note=Colocalizes with CABP1 and CIB1 at cell margin, membrane ruffles and punctate regions on the cell membrane. Colocalizes in adipocytes with GLUT4 in actin-based membranes. Localizes transiently at cell membrane to region known to be enriched in PIP2. Activation of phospholipase C results in its redistribution to the cytoplasm (By similarity) |
| Entrez Gene | 4641 |
|---|
Application
Western blot analysis of MYO1C Antibody (C-term) in K562 cell line lysates (35ug/lane). MYO1C (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MYO1C antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
