Application
Western blot analysis of PKMYT1 (arrow) using rabbit polyclonal PKMYT1 Center . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PKMYT1 gene.| Product Name | MYT1 (PKMYT1) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKMYT1; MYT1; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MYT1 |
|---|---|
| Alternative Names | PKMYT1 MYT1 Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase Myt1 kinase |
| Molecular Weight(MW) | 54521 Da |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development |
| Tissue Specificity | This MYT1 (PKMYT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136~166 amino acids from the center region of human PKMYT1. |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein |
| Entrez Gene | 9088 |
|---|
Application
Western blot analysis of PKMYT1 (arrow) using rabbit polyclonal PKMYT1 Center . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PKMYT1 gene.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
