Application
N4BP1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the N4BP1 antibody detected the N4BP1 protein (arrow).Application
N4BP1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the N4BP1 antibody (N-term)) for immunohistochemistry. Clinical relevance has not been evaluated.Application
N4BP1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | N4BP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | N4BP1; KIAA0615; NEDD4-binding protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0615 |
|---|---|
| Alternative Names | N4BP1 KIAA0615 NEDD4-binding protein 1 |
| Molecular Weight(MW) | 100379 Da |
| Function | Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity) |
| Tissue Specificity | Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary |
| Cellular Localization | Nucleus, nucleolus (By similarity). Nucleus, PML body (By similarity). Note=Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated (By similarity) |
| Entrez Gene | 9683 |
|---|---|
| Nucleotide Accession | NP_694574.3 |
Application
N4BP1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the N4BP1 antibody detected the N4BP1 protein (arrow).
Application
N4BP1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the N4BP1 antibody (N-term)) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
N4BP1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
