Application
NAE1 Antibody (Center) western blot analysis in K562,293 cell line lysates (35ug/lane).This demonstrates the NAE1 antibody detected the NAE1 protein (arrow).Application
NAE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NAE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NAE1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAE1; APPBP1; NEDD8-activating enzyme E1 regulatory subunit; Amyloid beta precursor protein-binding protein 1, 59 kDa; Amyloid protein-binding protein 1; Proto-oncogene protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APPBP1 |
|---|---|
| Alternative Names | NAE1 APPBP1 NEDD8-activating enzyme E1 regulatory subunit Amyloid beta precursor protein-binding protein 1 59 kDa Amyloid protein-binding protein 1 Proto-oncogene protein 1 |
| Molecular Weight(MW) | 60246 Da |
| Function | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation |
| Tissue Specificity | Ubiquitous in fetal tissues. Expressed throughout the adult brain |
| Cellular Localization | Cell membrane. Note=Colocalizes with APP in lipid rafts |
| Entrez Gene | 8883 |
|---|---|
| Nucleotide Accession | NP_001018170.1 NP_003896.1 |
Application
NAE1 Antibody (Center) western blot analysis in K562,293 cell line lysates (35ug/lane).This demonstrates the NAE1 antibody detected the NAE1 protein (arrow).
Application
NAE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NAE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
