Application
Western blot analysis of APP-BP1 (arrow) using rabbit polyclonal APP-BP1 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with APPBP1 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | APPBP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAE1; APPBP1; NEDD8-activating enzyme E1 regulatory subunit; Amyloid beta precursor protein-binding protein 1, 59 kDa; Amyloid protein-binding protein 1; Proto-oncogene protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APPBP1 |
|---|---|
| Alternative Names | NAE1 APPBP1 NEDD8-activating enzyme E1 regulatory subunit Amyloid beta precursor protein-binding protein 1 59 kDa Amyloid protein-binding protein 1 Proto-oncogene protein 1 |
| Molecular Weight(MW) | 60246 Da |
| Function | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation |
| Tissue Specificity | Ubiquitous in fetal tissues. Expressed throughout the adult brain |
| Cellular Localization | Cell membrane. Note=Colocalizes with APP in lipid rafts |
Application
Western blot analysis of APP-BP1 (arrow) using rabbit polyclonal APP-BP1 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with APPBP1 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
