Application
A. Western blot analysis on Hela cell lysate using anti-NAK/TBK1 (N-term) RabMAb (Cat. #AJ1525a), dilution 1:1,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- NAK/TBK1 (N-term) RabMAb (Cat. #AJ1525a).Application
C. Immunofluorescent staining of HeLa cells using anti- NAK/TBK1 (N-term) RabMAb (Cat. #AJ1525a).| Product Name | NAK/TBK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBK1, NAK, Serine/threonine-protein kinase TBK1, TANK-binding kinase 1;T2K;NF-kappa-B-activating kinase |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP611Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NAK |
|---|---|
| Alternative Names | TBK1 NAK Serine/threonine-protein kinase TBK1 TANK-binding kinase 1 T2K NF-kappa-B-activating kinase |
| Molecular Weight(MW) | 83642 Da |
| Function | Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFN- alpha and IFN-beta. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS or SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C Phosphorylates and activates AKT1. Phosphorylates Borna disease virus (BDV) P protein |
| Tissue Specificity | Ubiquitous with higher expression in testis. |
| Cellular Localization | Cytoplasm. Note=Upon mitogen stimulation or triggering of the immune system, TBK1 is recruited to the exocyst by EXOC2 |
| Entrez Gene | 29110 |
|---|
Application
A. Western blot analysis on Hela cell lysate using anti-NAK/TBK1 (N-term) RabMAb (Cat. #AJ1525a), dilution 1:1,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- NAK/TBK1 (N-term) RabMAb (Cat. #AJ1525a).
Application
C. Immunofluorescent staining of HeLa cells using anti- NAK/TBK1 (N-term) RabMAb (Cat. #AJ1525a).| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
