Application
Western blot analysis of NASP Antibody (C-term) in Hela cell line lysates (35ug/lane).NASP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with NASP antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | NASP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NASP; Nuclear autoantigenic sperm protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NASP Nuclear autoantigenic sperm protein |
|---|---|
| Molecular Weight(MW) | 85238 Da |
| Function | Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity) |
| Tissue Specificity | Isoform 1 is testis- and sperm-specific. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus. |
| Entrez Gene | 4678 |
|---|
Application
Western blot analysis of NASP Antibody (C-term) in Hela cell line lysates (35ug/lane).NASP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with NASP antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
