Application
Western blot analysis of NASP Antibody (N-term)) in HL-60 cell line lysates (35ug/lane).NASP (arrow) was detected using the purified Pab.Application
Western blot analysis of NASP (arrow) using rabbit polyclonal NASP Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2) .Application
NASP Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NASP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NASP; Nuclear autoantigenic sperm protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NASP Nuclear autoantigenic sperm protein |
|---|---|
| Molecular Weight(MW) | 85238 Da |
| Function | Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity) |
| Tissue Specificity | Isoform 1 is testis- and sperm-specific. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus. |
| Entrez Gene | 4678 |
|---|
Application
Western blot analysis of NASP Antibody (N-term)) in HL-60 cell line lysates (35ug/lane).NASP (arrow) was detected using the purified Pab.
Application
Western blot analysis of NASP (arrow) using rabbit polyclonal NASP Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2) .
Application
NASP Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
