Application
Western blot analysis of anti-NCE2 Antibody (N-term) in A375 cell line lysates (35ug/lane). NCE2(arrow) was detected using the purified Pab.Application
Western blot analysis of anti-NCE2 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). NCE2(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of NCE2 (arrow) using rabbit polyclonal NCE2 Antibody (C52) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCE2 gene.| Product Name | NCE2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2F; NCE2; NEDD8-conjugating enzyme UBE2F; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; Ubiquitin-conjugating enzyme E2 F |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NCE2 |
|---|---|
| Alternative Names | UBE2F NCE2 NEDD8-conjugating enzyme UBE2F NEDD8 carrier protein UBE2F NEDD8 protein ligase UBE2F NEDD8-conjugating enzyme 2 Ubiquitin-conjugating enzyme E2 F |
| Molecular Weight(MW) | 21077 Da |
| Function | Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5 |
| Tissue Specificity | Widely expressed (at protein level). |
Application
Western blot analysis of anti-NCE2 Antibody (N-term) in A375 cell line lysates (35ug/lane). NCE2(arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-NCE2 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). NCE2(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of NCE2 (arrow) using rabbit polyclonal NCE2 Antibody (C52) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCE2 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
