Application
NCK2 Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the NCK2 antibody detected the NCK2 protein (arrow).Application
NCK2 Antibody (N-term) (AP14107a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NCK2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCK2; GRB4; Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; SH2/SH3 adaptor protein NCK-beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRB4 |
|---|---|
| Alternative Names | NCK2 GRB4 Cytoplasmic protein NCK2 Growth factor receptor-bound protein 4 NCK adaptor protein 2 SH2/SH3 adaptor protein NCK-beta |
| Molecular Weight(MW) | 42915 Da |
| Function | Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum. |
| Entrez Gene | 8440 |
|---|---|
| Nucleotide Accession | NP_001004720.1 NP_003572.2 |
Application
NCK2 Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the NCK2 antibody detected the NCK2 protein (arrow).
Application
NCK2 Antibody (N-term) (AP14107a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
