Application
NCL Antibody (Center P291) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).Application
Fluorescent confocal image of U251 cell stained with NCL Antibody (Center P291)(Cat#AP14641c).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.| Product Name | NCL Antibody (Center P291) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCL; Nucleolin; Protein C23 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NCL Nucleolin Protein C23 |
|---|---|
| Molecular Weight(MW) | 76614 Da |
| Function | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats |
| Tissue Specificity | This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the Central region of human NCL. |
| Cellular Localization | Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 4691 |
|---|---|
| Nucleotide Accession | NP_005372.2 |
Application
NCL Antibody (Center P291) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).
Application
Fluorescent confocal image of U251 cell stained with NCL Antibody (Center P291)(Cat#AP14641c).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
