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NDUFA10 Antibody (Center)

Catalog Number:OM215115
OM215115
Discount Price
OM215115-100ul
1~2 Week
100ul
OM215115-50ul
50ul
OM215115-20ul
20ul
1~2 Week
Product Profile
Product Name NDUFA10 Antibody (Center)
Antibody Type Primary Antibodies
Antigen Alias NDUFA10; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; Complex I-42kD; NADH-ubiquinone oxidoreductase 42 kDa subunit
Key Feature
Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications WBIHCFC
WB:1:100~500 IHC
Species Reactivity Human
Concentration 1mg/ml
Target Information
Alternative Names NDUFA10
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10
mitochondrial
Complex I-42kD
NADH-ubiquinone oxidoreductase 42 kDa subunit
Molecular Weight(MW) 40751 Da
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Tissue Specificity This NDUFA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the Central region of human NDUFA10.
Cellular Localization Mitochondrion matrix.
Database Links
Entrez Gene 4705
Application

Application

NDUFA10 Antibody (Center) western blot analysis in HepG2 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NDUFA10 antibody detected the NDUFA10 protein (arrow).

Application

NDUFA10 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFA10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Application

NDUFA10 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes WB:1:100~500 IHC
Additional Information
Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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