Application
NDUFB5 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the NDUFB5 antibody detected the NDUFB5 protein (arrow).Application
NDUFB5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NDUFB5 Antibody (C-term)(Cat#AP11864b) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NDUFB5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDUFB5; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial; Complex I-SGDH; NADH-ubiquinone oxidoreductase SGDH subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NDUFB5 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 mitochondrial Complex I-SGDH NADH-ubiquinone oxidoreductase SGDH subunit |
|---|---|
| Molecular Weight(MW) | 21750 Da |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone |
| Tissue Specificity | This NDUFB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the C-terminal region of human NDUFB5. |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side |
| Entrez Gene | 4711 |
|---|---|
| Nucleotide Accession | NP_002483.1 |
Application
NDUFB5 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the NDUFB5 antibody detected the NDUFB5 protein (arrow).
Application
NDUFB5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NDUFB5 Antibody (C-term)(Cat#AP11864b) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
