Application
Western blot analysis of NDUFB8 (arrow) using rabbit polyclonal NDUFB8 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFB8 gene.Application
NDUFB8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFB8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NDUFB8 Antibody (Center)(Cat#AP11880c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NDUFB8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDUFB8; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; Complex I-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NDUFB8 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial Complex I-ASHI NADH-ubiquinone oxidoreductase ASHI subunit |
|---|---|
| Molecular Weight(MW) | 21766 Da |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone |
| Tissue Specificity | This NDUFB8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-95 amino acids from the Central region of human NDUFB8. |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side |
| Entrez Gene | 4714 |
|---|---|
| Nucleotide Accession | NP_004995.1 |
Application
Western blot analysis of NDUFB8 (arrow) using rabbit polyclonal NDUFB8 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFB8 gene.
Application
NDUFB8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFB8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NDUFB8 Antibody (Center)(Cat#AP11880c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
