Application
NDUFS1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NDUFS1 antibody detected the NDUFS1 protein (arrow).Application
NDUFS1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFS1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
NDUFS1 Antibody (Center) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NDUFS1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDUFS1; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; Complex I-75kD |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NDUFS1 NADH-ubiquinone oxidoreductase 75 kDa subunit mitochondrial Complex I-75kD |
|---|---|
| Molecular Weight(MW) | 79468 Da |
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized |
| Tissue Specificity | This NDUFS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human NDUFS1. |
| Cellular Localization | Mitochondrion inner membrane. |
| Entrez Gene | 4719 |
|---|---|
| Nucleotide Accession | NP_004997.4 |
Application
NDUFS1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NDUFS1 antibody detected the NDUFS1 protein (arrow).
Application
NDUFS1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFS1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
NDUFS1 Antibody (Center) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
