Application
A. Western blot analysis on (A) Hela and (B) K562 cell lysate using anti-NEDD8 RabMAb (Cat. #AJ1529a), dilution 1:500. (C) Western blot analysis of immunoprecipitation pellet from HeLa cell lysate using anti-NEDD8 RabMAb. (Cat. #AJ1529a), dilution 1:500.Application
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-NEDD-8 (N-term) RabMAb (Cat. #AJ1529a).| Product Name | NEDD8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEDD8, NEDD8, Ubiquitin-like protein Nedd8;Neddylin;Neural precursor cell expressed developmentally down-regulated protein 8 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y297 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEDD8 NEDD8 Ubiquitin-like protein Nedd8 Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 |
|---|---|
| Molecular Weight(MW) | 9072 Da |
| Function | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins |
| Tissue Specificity | Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes |
| Cellular Localization | Nucleus. Note=Mainly nuclear. |
| Entrez Gene | 4738 |
|---|
Application
A. Western blot analysis on (A) Hela and (B) K562 cell lysate using anti-NEDD8 RabMAb (Cat. #AJ1529a), dilution 1:500. (C) Western blot analysis of immunoprecipitation pellet from HeLa cell lysate using anti-NEDD8 RabMAb. (Cat. #AJ1529a), dilution 1:500.
Application
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-NEDD-8 (N-term) RabMAb (Cat. #AJ1529a).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
