Application
NEDD9 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NEDD9 antibody detected the NEDD9 protein (arrow).Application
NEDD9 Antibody (Center) (AP14592c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NEDD9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NEDD9 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEDD9; CASL; CASS2; Enhancer of filamentation 1; CRK-associated substrate-related protein; Cas scaffolding protein family member 2; Neural precursor cell expressed developmentally down-regulated protein 9; Renal carcinoma antigen NY-REN-12; p105; Enhancer |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CASL CASS2 |
|---|---|
| Alternative Names | NEDD9 CASL CASS2 Enhancer of filamentation 1 CRK-associated substrate-related protein Cas scaffolding protein family member 2 Neural precursor cell expressed developmentally down-regulated protein 9 Renal carcinoma antigen NY-REN-12 p105 Enhancer |
| Molecular Weight(MW) | 92861 Da |
| Function | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form |
| Tissue Specificity | Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues |
| Cellular Localization | Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle |
| Entrez Gene | 4739 |
|---|---|
| Nucleotide Accession | NP_006394.1 NP_892011.2 |
Application
NEDD9 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NEDD9 antibody detected the NEDD9 protein (arrow).
Application
NEDD9 Antibody (Center) (AP14592c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NEDD9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
