Application
Western blot analysis of NEIL1 Antibody (Center) in A2058 cell line lysates (35ug/lane). NEIL1 (arrow) was detected using the purified Pab.| Product Name | NEIL1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEIL1; Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; Nei-like protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEIL1 Endonuclease 8-like 1 DNA glycosylase/AP lyase Neil1 DNA-(apurinic or apyrimidinic site) lyase Neil1 Endonuclease VIII-like 1 FPG1 Nei homolog 1 Nei-like protein 1 |
|---|---|
| Molecular Weight(MW) | 43684 Da |
| Function | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Nucleus. Chromosome. Note=During mitosis, associates with centrosomes and condensed chromatin |
| Entrez Gene | 79661 |
|---|
Application
Western blot analysis of NEIL1 Antibody (Center) in A2058 cell line lysates (35ug/lane). NEIL1 (arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
