Application
NEIL3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NEIL3 antibody detected the NEIL3 protein (arrow).| Product Name | NEIL3 (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEIL3; Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEIL3 Endonuclease 8-like 3 DNA glycosylase FPG2 DNA glycosylase/AP lyase Neil3 Endonuclease VIII-like 3 Nei-like protein 3 |
|---|---|
| Molecular Weight(MW) | 67769 Da |
| Function | Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single- stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected |
| Tissue Specificity | Detected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue |
| Cellular Localization | Nucleus. |
| Entrez Gene | 55247 |
|---|---|
| Nucleotide Accession | NP_060718.2 |
Application
NEIL3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NEIL3 antibody detected the NEIL3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
