Application
The anti-NEK1 Pab is used in Western blot to detect NEK1 in CHO cell lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | NEK1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEK1; KIAA1901; Serine/threonine-protein kinase Nek1; Never in mitosis A-related kinase 1; Renal carcinoma antigen NY-REN-55 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1901 |
|---|---|
| Alternative Names | NEK1 KIAA1901 Serine/threonine-protein kinase Nek1 Never in mitosis A-related kinase 1 Renal carcinoma antigen NY-REN-55 |
| Molecular Weight(MW) | 142828 Da |
| Function | Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly |
| Tissue Specificity | High fetal expression in the brain and kidney. |
| Cellular Localization | Nucleus (Probable). Cytoplasm, cytoskeleton, centrosome (By similarity). Note=Associated with the pericentriolar material. Localizes to centrosome during interphase and mitosis (By similarity) |
| Entrez Gene | 4750 |
|---|
Application
The anti-NEK1 Pab is used in Western blot to detect NEK1 in CHO cell lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
